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Cloning Vector Bacteria

This post categorized under Vector and posted on June 11th, 2019.

A cloning vector need not contain suitable elements for the expression of a cloned target gene such as a promoter and ribosomal binding site (RBS) many however do and may then work as an expression vector.Cloning a cell means to derive a population of cells from a single cell. In the case of unicellular organisms such as bacteria and yeast this process is remarkably simple and essentially only requires the inoculation of the appropriate medium.Plasmid pET His6 MBP TEV LIC cloning vector (1M) from Dr. Scott Gradias lab. This plasmid is available through Addgene.

Researchers routinely use cloning techniques to make copies of genes that they wish to study. The procedure consists of inserting a gene from one organism often referred to as foreign DNA into the genetic material of a carrier called a vector. - Protocol .

A fragment of DNA containing the gene to be cloned is inserted into a suitable vector to produce a recombinant DNA molecule. The vector acts as a vehicle that transports the gene into a host cell usually a bacterium although other types of living cell can be used.Vector is an agent that can carry a DNA fragment into a host cell. If used for reproducing the DNA fragment it is called a cloning vector. If used for expressing certain gene in the DNA fragment it is called an expression vector.Note If you plan to phosphatase treat your cut vector it is necessary to use 5-phosphorylated oligos. This is an option that can be added when ordering them or can be performed enzymatically later.Gateway Recycling Cloning Ver. 2 Gateway Vector for Recycling Cloning Ver. 2 (pDONR201 backbone Km r for bacteria) Improvements in Ver.2 (1) Single digestion at RCS prior to LR reaction is enough for Ver.2 (Double digestion at RCS is necessary for Ver. 1).

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